Fig. 5. siRNA mediated knockdown of RhoG leads to inhibition of caspase-3 activity in INS-1 832/13 cells. Panel a: INS-1 832/13 cells were cultured in RPMI media containing basal glucose (2.5 mM) or high glucose (20 mM) for 24 hrs. Lysate proteins were resolved by SDS-PAGE and transferred to a nitrocellulose membrane and then probed for cleaved caspase-3. Uniform protein loading was ensured by re-probing the blots with antibody against β-actin. Representative blots from four independent studies are provided. Panel b: Densitometric quantitation of activated caspase-3 in Panel a is shown here. The results are shown as means ± SEM. The data are expressed as fold change relative to LG-mock (n=4; * p< 0.05). Comparisons shown: a - significant compared with HG-treated mock; b - significant compared with HG treated Cont-si.